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High Levels of Fluctuating Asymmetry in Isolated Stickleback Populations

08 October 2012

Fluctuating asymmetry (defined as small random deviations from the ideal bilateral symmetry) in isolated stickleback populations has been hypothesised to increase in response to both genetic and environmental stress experienced by a population, writes Nina Trokovic et al from the University of Helsinki.

Background

Three types of asymmetry in bilateral characters have been recognized: directional asymmetry, antisymmetry, and fluctuating asymmetry (FA). While both directional asymmetry (the same side is consistently larger) and antisymmetry (one of the sides is consistently larger) result from normal development, FA refers to subtle random deviations from perfect symmetry in bilateral traits resulting from developmental perturbations, and is often used as an indicator of stress and/or fitness e.g.. The assumption underlying this practise is that FA reflects developmental instability (DI) – an organism’s inability to adjust its development in an ideal symmetric pattern. Several studies have shown that high FA levels are characteristics of individuals with low fitness e.g.. The link between FA and various forms of stress has been repeatedly observed: habitat degradation , pollution , hybridisation, inbreeding, small population size, and marginal distribution have all been associated with increased levels of FA. Therefore, FA has been proposed to be a useful bioindicator of individual quality and/or environmental stress. However, despite these positive results, a number of studies have failed to find the expected relationships between FA and stress or fitness, fuelling a debate about the general applicability of FA as a bioindicator trait in conservation biology for reviews. Numerous analytical and statistical issues, such as the proper control of measurement error in metric traits, and the difficulty of reliably estimating DI using single traits, might provide at least partial explanation for the conflicting results. These difficulties have also been proposed to account for the recent decrease in popularity of FA studies.

While both theory and a number of observations align with the idea that the degree of FA at the individual or population level is indicative of individual quality or degree of stress experienced, relaxed selection against developmental perturbations is also expected to increase FA in given population and/or trait. For instance, several studies have shown that the levels of FA in functionally important bilateral traits is typically less than that in functionally less important traits. Similarly, it is possible that the degree of canalizing selection against developmental perturbations may differ among different populations. If so, this could provide one explanation for heterogeneity in FA-stress associations in different studies: in two populations experiencing the same incidence of stress induced developmental errors, the one experiencing relaxed selection against FA will express a higher degree of FA on average than a population that is under more stringent normalizing selection. However, to the best of our knowledge, this hypothesis has not been tested to date.

The mechanosensory lateral line system, present in all fishes and aquatic amphibians, has anatomical and functional properties which make it highly suitable and attractive for FA studies. Firstly, the lateral line system consists of numerous sensory receptors (neuromasts) located on the surface of the animal, either superficially (superficial or free neuromasts) or under the skin in fluid-filled canals (canal neuromasts), which can be counted easily. Meristic traits, such as neuromasts, have been shown to be superior over metric traits in detecting correlations between FA and the environment, and can be counted with little error. Secondly, neuromasts are organised in anatomically distinct lines that are distributed bilaterally along the head and trunk, and the existence of multiple traits (i.e. individual lines) provides the possibility to determine the overall level of FA precisely, unlike most single-trait estimations. Thirdly, the lateral line system is functionally very important, and likely to influence individual fitness. This system senses weak water movements and mediates crucial behaviours, including prey detection, predator avoidance, schooling, orientation to water currents (rheotaxis), and localization of objects. Hence, lateral line asymmetry is likely to reduce fitness, and as such, it is a potential target of natural selection. Further, this effect could be expected to differ among populations living in environments which differ in the demands on the lateral line system.

The goal of the present paper was to compare the degree of FA of marine (high piscine predation risk) and pond (zero piscine predation risk) nine-spined stickleback (Pungitius pungitius) populations differing both in the levels of genetic diversity and in the level of expected selection (by piscine predation) for symmetry. Assuming that perfect symmetry in the lateral line system is favoured by natural selection, we hypothesised that either (i) the relaxed selection for symmetry in pond populations under negligible predation, and/or (ii) the reduced genetic variability (= genetic stress) in pond populations, will result in reduced developmental stability in ponds as compared to marine populations. In both cases, one would expect to see higher FA levels in pond than in marine populations. However, because genetic variability varies between populations within the same habitat [35], we also attempted to disentangle the two alternative explanations for increased FA in pond environments.

Results

The GLMM on heterozygosity revealed a significant habitat effect (F1,6 = 10.17, P = 0.019), but no population effect (Z = 1.45, P = 0.15). The average (± S.E.) heterozygosity in marine populations (HE = 0.58 ± 0.06) was approximately two times higher than in pond populations (HE = 0.30 ± 0.06; Figure 1). The GLM revealed a significant population effect (F7,176 = 14.47, P < 0.001) and subsequent post hoc tests revealed no heterogeneity among marine populations (all P > 0.22, Figure 1). The Mashinnoje pond population that has only recently become isolated from the White Sea; White Sea Biological Station staff personal communication] did not differ from the marine populations in terms of heterozygosity (all P > 0.13, Figure 1). The remaining three ponds (Abbortjärn, Pyöreälampi and Rytilampi) had lower heterozygosity than the marine or Mashinnoje populations (all P < 0.01, Figure 1). Pyöreälampi had lower heterozygosity than any of the other populations (all P < 0.004, Figure 1). Therefore, while the marine populations had uniformly high heterozygosity, in the ponds heterozygosity varied from being similar to the marine levels (Mashinnoje) to almost zero (Pyöreälampi; Figure 1.).

The GLMM revealed a significant habitat effect (F1,6 = 35.60, P < 0.001) on the composite standardized relative FA-index, irrespective of sex (F1,156 = 0.27, P = 0.60), population (Z = 1.05, P = 0.30), and heterozygosity (F1,5 = 2.19, P = 0.20). None of the interactions were significant (habitat*sex: F1,143 = 0.03, P = 0.86; habitat*heterozygosity: F1,4 = 0.10, P = 0.76). The pond populations showed almost three times higher levels of asymmetry than the marine populations (Least Squares means ± S.E.; marine = 6.55 ± 1.3; pond = 17.45 ± 1.3; Figure 1). The multivariate GLM supported these results, revealing a strong habitat effect (Wilk’s lambda 12,141 = 0.59, P < 0.001) on standardized relative asymmetry, irrespective of sex (Wilk’s lambda 12,140 = 0.91, P = 0.36, habitat*sex: Wilk’s lambda 12,139 = 0.96, P = 0.91). The population effect was also non-significant (Wilk’s lambda 72,773 = 0.54, P = 0.07), and the univariate tests showed significant habitat effects for standardized relative asymmetry in all 12 traits (F1,150 > 5.38, P < 0.02). The trends were similar to those revealed by the composite relative asymmetry index: pond populations were generally more asymmetric than marine populations (data not shown).

To explore further the (lack of) heterozygosity effect on FA in the GLMM (see above), we performed simple correlation analyses using the population mean values of the two traits. Using raw values, there was strong negative correlation between mean FA and mean heterozygosity across the eight populations (rs = - 0.833, P = 0.01). However, if the average effect of habitat type is controlled for by performing the correlation using heterozygosity values standardized to a common mean across the habitat types, this correlation disappears (rs = - 0.071, P = 0.35). These results are compatible with the results of the GLMM above, and show that the association between FA and heterozygosity is mainly driven by the association between habitat type and heterozygosity.

Discussion

The most salient finding of this study was that nine-spined sticklebacks from ponds exhibit significantly and consistently higher levels of FA than their marine conspecifics. Furthermore, while the pond sticklebacks in general had only about half of the genetic variability of marine sticklebacks, the analyses did not support the idea that habitat differences in levels of FA are explainable by differences in heterozygosity once the habitat differences in heterozygosity are controlled for. Hence, the results support the conjecture that high levels of FA in pond populations stem from decreased selection for perfect symmetry, rather than from genetic stress.

Predation is a widely recognized mechanism of natural selection, and some studies have shown that predated individuals express higher levels of FA than surviving individuals e.g. [9,37-39]. Furthermore, decreased FA with age is also suggestive of poorer survival of more asymmetric individuals e.g.[40]. Predation can decrease the population level FA in the prey in at least three ways. First, predation can impose selection for individuals with low FA, resulting in a high degree of developmental canalization and thereby in low FA. However, this implies that there is an additive genetic basis for FA. Heritability of FA is a controversial issue: initial meta-analyses yielded a relatively high average heritability estimate [41], but the subsequent studies have since suggested that the heritability of FA is very low – if not negligible. Second, assuming that FA has no, or a weak, genetic basis, and is simply a reflection of the growth environment experienced, predation might simply remove asymmetric individuals from the population. Third, as negligible predation selects for larger body size and higher growth rate predation has the potential to affect FA indirectly through altering the growth intensity, where higher growth rate is coupled with higher DI . Regardless of the mechanism, relaxation of predation pressure can be expected to increase the average degree of FA in the population. While we are not aware of any studies that have compared FA levels among populations that differ in selection for perfect symmetry, there are studies which show that less functionally important traits express higher FA than important traits at the individual level. That said, it is also known that strong directional selection can increase DI in selected traits. However, although the mean number and organisation of lateral-line neuromast differ among marine and pond populations in this species, the patterns of differentiation among populations are heterogeneous and directional selection on lateral-line traits is indicated to occur mainly in the marine environment. Likewise, the lateral plate number – one of the traits analysed in study – is shown to be reduced in pond as compared marine populations presumably as response relaxed piscine predation in pond environments. Hence, it seems unlikely that the increased DI in pond populations’ lateral-line traits and lateral plate numbers would results from directional selection.

Perhaps the most marked difference between pond and marine nine-spined stickleback populations is the predation risk; marine sticklebacks are sympatric to a large number of predatory fish species, while ponds lack predatory fish and the nine-spined stickleback is often the only fish species present in ponds. Previous studies have demonstrated marked behavioral and morphological differences in nine-spined sticklebacks in relation to predation risk, including a recent study demonstrating habitat and population specific differences in the lateral line system. The mechanosensory lateral line system of fish and aquatic amphibians responds to weak water movements and is involved in avoidance of predators and in schooling, which is an important antipredator behaviour. Hence the negligible predation in ponds might have resulted in relaxed selection for perfect symmetry in the lateral line system and consequently, in the high levels of FA observed in this habitat. While this is, to the best of our knowledge, the first study to suggest this effect, we admit that the exact functions of the different lateral-line traits in this species are as yet unknown. Hence, further functional studies about how the information from the lateral-line is used in different contexts are needed. However, the fact that levels of FA in lateral plate numbers – a trait associated with variation in piscine predation – showed exactly the same patterns of FA as lateral-line traits supports the importance of predation in dictating the observed patterns..

Inbreeding (mating among relatives) can increase homozygosity and result in inbreeding depression, which can manifest itself in reduced survival and fertility Increased FA levels have been linked to reduced heterozygosity both in the field , and in controlled laboratory experiments with induced inbreeding. It has been shown that pond nine-spined stickleback populations have lower genetic variability than marine populations, and one explanation for the higher level of FA in pond sticklebacks could be that reduced genetic variability in pond populations has resulted in increased DI, and consequently increased FA. Based on the populations used in this study, the heterozygosity of pond populations was on average half that of marine populations, with heterozygosity being highly variable among pond populations, but similar among marine populations. However, formal tests – accounting for the on average lower heterozygosity in pond populations – failed to find association between heterozygosity and FA across the populations. This finding is not completely surprising, as some other studies also found that heterozygosity had a weak, or no effect on FA. However, given the fact heterozygosity and habitat type are tightly associated in our study, their independent effects on FA cannot be fully disentangled.

Obviously, there are other factors that potentially affect FA that we could not directly address here. For instance, there might be environmental stressors (e.g. water quality, temperature, oxygen levels, pH, etc.) that may differ among marine and pond populations, and cause higher levels of FA in ponds. At the moment, too little is known about the variation in relevant environmental parameters and their potential impact on FA in pond vs. marine habitats to form informed arguments about their significance, but it is worth noting that there is no a priori reason to suggest that pond fish would experience more stressful environmental than the marine fish. In fact, pond fish live longer, grow faster and attain larger sizes than marine fish both in laboratory and the wild. Nevertheless, more environmental data coupled with experiments conducted under common garden settings would be needed to study possible environmental determinants of high FA in pond fish. However, irrespectively of the causes, the fact remains that the levels of FA in pond populations are markedly elevated as compared to marine populations.

Conclusions

In conclusion, the results demonstrate that there is a three-fold difference in levels of FA between pond and marine nine-spined stickleback populations (pond > marine). While there is also a two-fold difference in heterozygosity (pond < marine), the loss of genetic variation did not explain the divergence in levels of FA once the habitat differences in heterozygosity were controlled for. We hypothesize that the negligible predation in pond populations (contrasted to the high predation in marine environments) is responsible for the increased FA in ponds.

October 2012

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