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Effect of Incorporating “OXYDOL®” in White Shrimp Farming Ponds Over Production and Physical-Chemical Parameters

26 May 2014

Marcelo Atehortua Herrera , Zootechnical Veterinarian Doctor (BIOPHARM S.A. ) looks at the effect of incorporating “OXYDOL®” in white shrimp farming ponds (Litopenaeus vannamei) over production and physical-chemical parameters.



  • Amount of oxigen dissolved
  • Evaporation and filtering
  • Water temperature
  • Coloring
  • Salinity
  • Turbidity
  • pH


Water temperature shall be between 25 and 32°C, being the optimal between 29 and31°C (Yoong Basurto y Reinoso Naranjo, 1982).


It can range between 28 and33 ppt (parts by thousand) (Cuellar, Lara, Morales, De Gracia 2010).


Normal concentrations range between 4 and 7 ppm (mg/L).


Optimal pH range is between 7.0 y 8.0 (Cuellar, Lara, Morales, De Gracia 2010).

In the ponds this element comes from the replacement water, photosynthesis and at a lesser degree than the one dissolved on the pond’s surface coming from the atmosphere. Lower concentrations of oxygen are oberserved at dawn and greater ones at the end of the day. Normal concentration ranging between 4 and 7 ppm (mg/L) are considered.

It must be pointed out that the oxygen ponds have a tendency to stratify, that is that there is generally a greater concentration on the superior layer of the water, than at the bottom; because the shrimps live there, it is necessary to make an homogenization of the water column to obtain a correct aeration.

The optimal range of pH is between 7.0 and 8.0 but the pH 5 values have shown not be harmful for shrimps. Notwithstanding, a pronounced increase or decrease of the pH values can cause lethal effects to the ecological balance of the pond.


Must be greater than 30cm.


Pale green: Indicates adequate concentration of algae

Gray: Few algae in the pond, it is recommended more fertilization and replacemente of water.

Moss green: Algae starts dying, it is required urgent need of water replacement.

Brilliant green: High concentration of algae.

Brown: High amount of dead algae, probably there is lack of nutrients and excess of metabolites.

It gives an idea of the material pending on the pond’s water, this material interferes in the light’s passage. The turbidity is measured with a Secchi disc and it is the depth measure into which this disc disappears upon submerging it in the water. If visibility is lower than 30 cm, there are potential problems, if light is greater, it can better penetrate and there will be more productivity and growth of the organisms from which the shripm can be fed.


Take into consideration:


It is convenient to feed the animals twice a day, in the morning and in the afternoon..

because if you provide the feed at once, it will not be consummed inmediately and therefore it will start decomposing, originating not only contamination but also a decrease on the concentration of dissolved oxygen, mainly at the bottom of the pond.


Starts at 25 to 32 lb/ha/day with a 25 to 35% protein (Dirección Nacional de Acuicultura Panamá, 1984; CAMACO, 2013).


In general, penaeid live at soft mud bottoms, made by different proportions of sand, slime and clay, burying themselves and protecting from predators, mainly during the molting period; this behavior seems to be regulated by factors such as light, temperature and concentration of oxygen.

In general penaid live at soft mud bottoms, made by different proportions of sand, lime and clay. Specie such as Penaeus duorarum, P.japonicus, P.aztecus, P.setiferus, P.vannamei y Pleoticus muelleri bury themselves proctecting from predators, mainly during the molting period; this behavior seems to be regulated by factors such as light, temperature and concentration of oxygen.


  • Carbon added is transformed in form of food or organic and inorganic fertilization, due to the participation of physical, chemical and biological activities.
  • Intensive production is accompanied by increase of carbon input as food, that in form of food exceeds the metabolic capacity of the pond, resulting on the accumulation of organic composites and deterioration to quality of the water.
  • The greater output of carbon from the productive system is associated with the escape of gas in form of CO2. The main means of CO2 retention in the fish ponds is the phyoplankton.

Some research shows that the quality of water has become the main limitation in the search for a greater intensification of farm fish production.

  • Carbon in ponds is determined by the type of food, feeding practices, specie farmed, water replacemenet, aeration, depth of the pond and living microorganisms.
  • Carbon, together with nitrogen and phosporus are required in greater quantity at the different physical chemical reactions in the ponds.
  • In the different metabolic processes occurring inside the ponds, carbon participates practically in all reactions and is the most abundant of all the organic molecules (Boyd, 1995).


Recurrent application of food into the ponds, together with shrimps and other species’ debry from the system itself, originate that during productive cycle the organic material accumulates in the bottom of the ponds and that along the years the soils degrade.

As a result of this vicious circle:

  • quality of water deteriorates
  • to control it, it is not possible to mecanically make a sweep or cleaning of the ponds’ bottoms( favoring the occurrence of bacteria and opportunistic virus that end up affecting the population’s health in the lakes.)
  • major economical loses to corporations occurr as a result of the foregoing.


  • Product designed based upon specific beneficial bacteria, enzymes, organic catalitics, stabilizers and potentiators for degradation of the organic material in the bottom of lakes used in intensive production.
  • A bio secured alternative to improve aquicultural ecosystems and the physical chemical quality of water in production activities.

OXYDOL®. Benefits

  • Accelerates the degradation process and decresing organic waste.
  • Controls proliferation of algae in the environment
  • Allows oxygenation of the environment, without competing with other bacteria.
  • Decreases concentration of ammonia, methane and sulphites in water.
  • Restores the aquicultural chemistry by natural means increasing the shrimps’ survivorship in ponds.
  • Allows conducting specific works in ponds such as:

- Death corners, oxygen problems, control of algae growth, treatment of charged waters, coloring, pigmentation in shrimps, significant decrease of odors and tastes.


  • The trial took place in Panama, with an important shrimp company, to evaluate the effect OXYDOL® over the main production variables in the farm.
  • Evaluation conducted during a complete cultivation cycle between May and November 2012 in 10 ponds, of area 700-A in the area of Aguadulce.


  • To bio-remedy the bottom of the lake for intensive shrimp production:

- Improving the physical chemical parameters of water;

- Controlling emission of harmful gas;

- Promoting adequate sanity, sustainability, zootechnical production;

- Increasing profitability of aquicultural companies.


Shrimps and experimental units

Shrimps used in the trial were obtained at the Larva Production Center “C.P.L. San Carlos” (San Carlos, Panama) and were submitted to an acclimatization process of 10 days, then transferred to 10 experimental ponds at state of post-larva 22 (pl-22)

10 pounds were used (5 with OXYDOL® and 5 as Control) of 3 Has. each one in the area 700-A of the farm, said ponds were selected by similarity of size, type of bottoms, filling water and replacement water.


The trial was conducted based upon a design of blocks totally at random, grouping ponds to form two treatments:

  • OXYDOL ® treatment ponds 721 to 725 (5 replicas)
  • Control treatment: ponds 716 to 720 (5 replicas)


  • Physical chemical parameters (dissolved oxygen [OD in mg/L], temperature [in °C], salinity [in ppt] and pH)
  • Survivorship (%)
  • Production in pounds by hectare (lb/ha)
  • Food conversion factor (FC)
  • Weight of harvest (Weight g)
  • Weekly growth (Growth gr)
  • Cost-benefit relationship ($)


  • A preparation and filling protocol of the 10 ponds was routinaley used in the farm. Shrimps were planted in all the ponds with an average density of 15 15 pls/m2. Fertilization in water was made with ammonia Nitrate, according with the farm’s protocol (15-25 lb/ha).
  • The post-larva pl-22, were fed during the whole cycle (200-209 days) twice a day by scattering standarized diet for both treatments, using “witnesses feeders” as guides. The ponds were kept closed with no water replacement during the first 30 cultivation days.

OXYDOL®, Application protocol

Dilute 1 Kilogram of OXYDOL® in 19 liters of water (free of chlorine)


  • Weekly sampling of weight, data of physical chemical parameters was collected in the farm according to protocol for each one of them ( (OD and T, twice a day; salinity and pH once a week)
  • Production values were provided by ALTRIX DE PANAMA processing plant.
  • Data was statistically analyzed by ANOVA, Duncan y Chi trials aligned with the SAS program, considering the differences as signicant when P≤ 0.05. The Chi trial aligned was applied only to FC, for being a non parametric variable.


  • During cultivation were shown outbreaks caused by virus WSSV (white spot sindrome) according with expectations in the 01 experimen-tal ponds, which was confirmed through polimerasa (Nested-PCR) chain tests reaction and hystopathology which in certain degree influenced in the final zootechnical parameters.
  • Based upon these disease outbreakes, it is suggested that there was a positive tendency from using OXYDOL® over the shrimps’s survivorship, which showed a highly significant difference (P<0.05).


  • Cost of OXYDOL® in this trial was of $62.50 /ha. Considering that 15 hectare were treated (5 ponds at 3 hectares each one), the total cost of the product in the trial was of $937.50
  • To cover the cost of OXYDOL® in this trial and having a base price of $2.20 per lb of shrimp, it was required to produce 28.4 lb/ha more in the ponds with OXYDOL®, each hectare with OXYDOL® produced in average 389 pounds more than the Control.
  • The product paid itself ~14 times.
  • The majority of probiotics micro-organisms proposed for aquiculture belong to the acid-lactic bacteria (LAB), of which the genre more used are Lactobacillus and Lactococcus. These are considered as GRAS (“Generally recognized as safe”), a warranty that the implementation of isolated probotics will not cause collateral damage to the cultivated organisms or to the final consumers. Institute of Marine and Coastal Research INVEMAR 173; (Holzapfel et al., 1998).
  • Use of probiotics as LAB is relatively well established in other animal specie (Wallace y Newbold, 1992; Aiba et al., 1998; Kontula et al., 1998; Kirjavainen et al., 1999a, 1999b; Netherwood et al., 1999), of them it oustands the increase on height and weight, establishment of an intestinal microbial equilibrium, as well as the improvement of some inmune responses.
  • Supply of exogenous LAB is also associated with the growth inhibition of the pathogen bacteria (Lewus et al., 1991; Gildberg et al., 1995; Santos et al., 1996), as growth promoter of the aquiculture specie (Noh et al., 1994) and in some cases, with an increase of shrimps’ survivorship experimentally infected (Gatesoupe, 1994; Gildberg, 1995; Robertson et al., 2000).
  • Utilization of Gram-positive bacteria such as Bacillus, in general has shown to be beneficial in shrimp cultivation (L. vannamei), causing an important increase in the rates of survivorship and weight. (Jory, 1998).
  • Bacteria of the Bacillus genre selected as probiotics can convert organic matter into O2 , (Dalmin et al., 2001). Laloo et al. (2007) proved the capability of three isolated of the Bacillus genre to decrease concentration of nitrite, nitrate and ammonia in the cultivation water of ornamental fish. This same phenomena was also observed by Kim et al. (2005) en B. subtilus, B.cereus y B. licheniformis.
  • Interaction between probiotic bacteria and micro algae in the cultivation ponds, in general produces positive effects, since it estabilizes the nutritional factors of the living feed, being able to contribute with the establishment of the beneficial intestinal micro flora of the host (Reitan et al., 1993, 1997).


  • OXYDOL® treatment exceeded control in 3 of the 5 production variables with higher economical impact.: “survivorship”, “lb/ha” and“FC”. The first two showed significant differences (P<0.01). The variables “harvest weight” and “weekly growth”, were better at control, without showing signifcant differences. (P < 0.05).
  • Cost of OXYDOL® used in this trial paid itself 14 times, based upon the incremental pounds of shrimps obtained regarding the Control.
  • This difference of lb/ha obtained in favor of the product, suggests that keeping a consistent production system of cultivation shrimp could represent an economical increase.

May 2014

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