CANADA - University of Alberta scientists are working to mitigate the risks of toxic algae blooms using advanced flow cytometry, a technique they developed and have been using for the past three years.
As we approach the hottest days of the summer season, toxic algae are starting to bloom in lakes around the world.
Increases in atmospheric temperature combined with land nutrients are promoting the growth of harmful cyanobacteria - blue-green algae - in these bodies of water, damaging for not only the associated ecosystems but also potentially for the people who recreationally frequent the lakes.
"Our ability to rapidly detect the onset of outbreaks of potentially toxic cyanobacteria provides timely information to the public regarding the major lakes throughout the province," said Professor Rolf Vinebrooke (Biological Sciences).
"If the cell counts exceed the World Health Organization guideline of 100,000 cells per millilitre, we notify Alberta Health officials, who then post advisories at the lake in question."
Vinebrooke and his colleagues use a "FlowCAM" unit, manufactured by Fluid Imaging Technologies, for early detection of cyanobacteria in lakes.
Their protocol is the first adaptation in North America of the protocol used by oceanographers to warn of potential shellfish poisoning events produced by toxic marine algae.
A sample of lake water is simply allowed to flow through the cytometer, which is equipped with imaging software that detects and measures more than 25 different optical properties of each cell.
At the same time, the software constructs a collage of all the images and then matches each with a known cyanobacterium using a searchable electronic library of more than 100 species, developed by research associate Dr Mark Graham.
"The next step is to detect trends in our cyanobacterial-monitoring data as related to measured environmental conditions," continues Mr Vinebrooke.
"This will enable us to generate both predictive models for blue-green algal blooms and testable hypotheses regarding the as-yet unknown set of factors that cause these outbreaks in the first place."
Mr Vinebrooke recently collaborated with colleagues from McGill University to quantitatively synthesise long-term worldwide trends of cyanobacterial growth.
The scientists found that, as humans have altered the global climate over the past two centuries, there has been a disproportionate growth of blue-green algae relative to other phytoplankton, with particularly accelerated growth in the past 70 years.
"Excess inputs of nutrients into lakes combined with higher water temperatures due to climate warming appear to be two of the major causes for the outbreaks," he said.
"We recommend reducing the use of fertilisers and fossils fuels to help slow the global acceleration of this cyanobacterial dominance."
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